245

Title

A genomic amplification affecting a carboxylesterase gene cluster confers organophosphate resistance in the mosquito Aedes aegypti: from genomic characterization to high-throughput field detectionuse asterix (*) to get italics

Authors

Julien Cattel, Chloé Haberkorn, Fréderic Laporte, Thierry Gaude, Tristan Cumer, Julien Renaud, Ian W. Sutherland, Jeffrey C. Hertz, Jean-Marc Bonneville, Victor Arnaud, Camille Noûs, Bénédicte Fustec, Sébastien Boyer, Sébastien Marcombe, Jean-Philippe DavidPlease use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"

Year

2020

Picture

Abstract

<p>By altering gene expression and creating paralogs, genomic amplifications represent a key component of short-term adaptive processes. In insects, the use of insecticides can select gene amplifications causing an increased expression of detoxification enzymes, supporting their usefulness for monitoring the dynamics of resistance alleles in the field. In this context, the present study aims to characterise a genomic amplification event associated with resistance to organophosphate insecticides in the mosquito *Aedes aegypti* and developing a molecular assay to monitor the associated resistance alleles in the field. An experimental evolution experiment using a composite population from Laos confirmed the association between the over-transcription of multiple contiguous carboxylesterase genes on chromosome 2 and resistance to multiple organophosphate insecticides. Combining whole genome sequencing and qPCR on specific genes confirmed the presence of a ~100 Kb amplification spanning at least five carboxylesterase genes at this locus with the co-existence of multiple structural duplication haplotypes. Field data confirmed their circulation in South-East Asia and revealed high copy number polymorphism among and within populations suggesting a trade-off between this resistance mechanism and associated fitness costs. A dual-colour multiplex TaqMan assay allowing the rapid detection and copy number quantification of this amplification event in *Ae. aegypti* was developed and validated on field populations. The routine use of this novel assay will improve resistance tracking in this major arbovirus vector.</p>

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Keywords

genomic amplification, insecticide resistance, mosquito, Aedes aegypti, carboxylesterase, diagnostic assay

Methods that require specific expertise (optional)

NonePlease indicate the methods that may require specialised expertise during the peer review process (use a comma to separate various required expertises).

Thematic fields

Adaptation, Evolutionary Applications, Experimental Evolution, Genome Evolution, Molecular Evolution

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Submission date

2020-06-09 13:27:18

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Stephanie Bedhomme

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