Trait-specific trade-offs prevent niche expansion in two parasitesuse asterix (*) to get italics

Eva JP Lievens, Yannis Michalakis, Thomas LenormandPlease use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"

2019

<p>The evolution of host specialization has been studied intensively, yet it is still often difficult to determine why parasites do not evolve broader niches – in particular when the available hosts are closely related and ecologically similar. Here, we used an experimental evolution approach to study the evolution of host specialization, and its underlying traits, in two sympatric parasites: *Anostracospora rigaudi* and *Enterocytospora artemiae*, microsporidians infecting the brine shrimp *Artemia franciscana* and *Artemia parthenogenetica*. In the field, both parasites regularly infect both hosts, yet experimental work has revealed that they are each partially specialized. We serially passaged the parasites on one, the other, or an alternation of the two hosts; after ten passages, we assayed the infectivity, virulence, and spore production rate of the evolved lines. In accordance with previous studies, *A. rigaudi* maintained a higher fitness on *A. parthenogenetica*, and *E. artemiae* on *A. franciscana*, in all treatments. The origin of this specialization was not infectivity, which readily evolved and traded off weakly between the host species for both parasites. Instead, the overall specialization was caused by spore production, which did not evolve in any treatment. This suggests the existence of a strong trade-off between spore production in *A. franciscana* and spore production in *A. parthenogenetica*, making this trait a barrier to the evolution of generalism in this system. This study highlights that the shape of between-host trade-offs can be very heterogeneous across parasite traits, so that only some traits are pivotal to specialization.</p>

https://zenodo.org/record/3476544You should fill this box only if you chose 'All or part of the results presented in this preprint are based on data'. URL must start with http:// or https://

https://www.biorxiv.org/content/10.1101/621581v4.supplementary-materialYou should fill this box only if you chose 'Scripts were used to obtain or analyze the results'. URL must start with http:// or https://

Ecological specialization, niche, host specificity, experimental evolution, parasite life history, multi-host parasites, Artemia, microsporidians

NonePlease indicate the methods that may require specialised expertise during the peer review process (use a comma to separate various required expertises).

Adaptation, Evolutionary Ecology, Evolutionary Epidemiology, Experimental Evolution, Life History, Species interactions

2019-05-13 13:44:34

Frédéric Guillaume