Community structure of heritable viruses in a *Drosophila*-parasitoids complexuse asterix (*) to get italics

Julien Varaldi, David Lepetit, Nelly Burlet, Camille Faber, Bérénice Baretje, Roland AllemandPlease use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"

2024

<p style="text-align: justify;">The diversity and phenotypic impacts related to the presence of heritable bacteria in insects have been extensively studied in the last decades. On the contrary, heritable viruses have been overlooked for several reasons, including technical ones. This is regrettable because of the size of this gap knowledge and because case study indicate that viruses may have profound impact on the functionning of individuals and communities. Additionally, the factors that may shape viral communities are poorly known, except in some very specific viral-insect systems. Here we analyze the community structure of heritable viruses in a multi-hosts-multi-parasitoids community. Drosophilidae and their larval and pupal parasitoids were sampled in two locations, two years and two seasons. After two lab generations, putative DNA and RNA viruses were purified and sequenced. Our analysis revealed the presence of 53 viruses (including 37 new viruses), the great majority of which were RNA viruses. The "species" factor was by far the most significant one, explaining more than 50% of the variance in viral structure. Additionally, parasitoids had a higher number of heritable viruses compared to their hosts, suggesting that this lifestyle favours the association with viruses. Finally, our community-level survey challenged previous interpretation concerning the host range of some previously described viruses.</p> <p>&nbsp;</p>

https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1000623You should fill this box only if you chose 'All or part of the results presented in this preprint are based on data'. URL must start with http:// or https://

https://github.com/jVaraldi/Viromics/tree/mainYou should fill this box only if you chose 'Scripts were used to obtain or analyze the results'. URL must start with http:// or https://

virus; community; insect; parasitoid; symbiont; superparasitism

NonePlease indicate the methods that may require specialised expertise during the peer review process (use a comma to separate various required expertises).

Evolutionary Ecology, Species interactions

Darren Obbard [darren.obbard@ed.ac.uk], Lena Wilfert [lena.wilfert@uni-ulm.de], Lena Wilfert [lena.wilfert@uni-ulm.de] suggested: Dear Ben,, Lena Wilfert [lena.wilfert@uni-ulm.de] suggested: really sorry to decline, but I'm off on holidays then on to conferences from tomorrow. All the best for the RES meeting, maybe see you in Roscoff? Hope you're all doing great!, Lena Wilfert [lena.wilfert@uni-ulm.de] suggested: You know the community better than me, but maybe Elisabeth Herniou or someone like that?, Lena Wilfert [lena.wilfert@uni-ulm.de] suggested: cheers, Lena Wilfert [lena.wilfert@uni-ulm.de] suggested: Lena, Francis Jiggins suggested: Sorr, just have rather a lot of reviewing at the moment. Maybe you can persuade Darren Obbard?, Robert L. UNCKLESS [unckless@ku.edu] suggested: I’m really sorry, but I’m behind on overyrhing right now. Try Andrew Routh - alrouth@utmb.edu No need for them to be recommenders of PCIEvolBiol. Please do not suggest reviewers for whom there might be a conflict of interest. Reviewers are not allowed to review preprints written by close colleagues (with whom they have published in the last four years, with whom they have received joint funding in the last four years, or with whom they are currently writing a manuscript, or submitting a grant proposal), or by family members, friends, or anyone for whom bias might affect the nature of the review - see the code of conduct

2023-08-03 01:07:43

Ben Longdon