Cross-tolerance evolution is driven by selection of heat tolerance in *Drosophila subobscura*use asterix (*) to get italics

Luis E. CastañedaPlease use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"

2024

<p>The evolution of heat tolerance is a crucial mechanism for the adaptive response to global warming, but it depends on the genetic variance carried by populations and on the intensity of thermal stress in nature. Experimental selection studies have greatly benefited research into heat tolerance, providing valuable insights into its evolutionary process. However, the impact of varying levels of heat stress intensity on the associated changes in resistance traits has not yet been explored. Here, the correlated evolution of increasing knockdown temperature in <em>Drosophila subobscura</em> was evaluated on the knockdown time at different stress temperatures (35, 36, 37, and 38 ºC), thermal death time (TDT) curves, and desiccation and starvation resistance. The selection of heat tolerance was performed using different ramping temperatures to compare the impact of heat intensity selection on resistance traits. Correlated evolution was found for these four resistance traits in <em>D. subobscura</em>, indicating that the evolutionary response to tolerance of higher temperatures also confers the ability to tolerate other stresses such as desiccation and starvation. However, these correlated responses depended on the intensity of thermal selection and sex, which may limit our ability to generalize these results to natural scenarios. Nevertheless, this study confirms the value of the experimental evolutionary approach for exploring and understanding the adaptive responses of natural populations to global warming.</p>

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correlated evolution, global warming, heat stress intensity, stress resistance 30 evolution, thermal tolerance landscape.

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Adaptation, Experimental Evolution

Pedro Simoes (pmsimoes@fc.ul.pt), Julián Mensch (julianmensch@gmail.com ), Patricia Beldade (pbeldade@fc.ul.pt), Jens Rolff suggested: Volker Loeschke or Sophie Armitage would be suitable reviewers. , Jens Rolff suggested: I apologize for not being able to review this, but I am travelling and will then teach a solid block course., Max Reuter suggested: Neda Barghi, Vetmed Uni Vienna, barghi.neda@gmail.com, Matthew Sasaki suggested: James DeMayo - JAMES.DEMAYO@ucdenver.edu, James deMayo [james.demayo@ucdenver.edu] suggested: Ioulia Bespalova (ioulia.bespalova@gmail.com), Julien Foucaud suggested: Laure Olazcuaga : olaz.laure@gmail.com , Kathrin Otte [kotte@uni-koeln.de] suggested: Neda Barghi Neda.Barghi@vetmeduni.ac.at, Laure Olazcuaga [olaz.laure@gmail.com] suggested: Sara Magalhães: snmagalhaes@fc.ul.pt No need for them to be recommenders of PCIEvolBiol. Please do not suggest reviewers for whom there might be a conflict of interest. Reviewers are not allowed to review preprints written by close colleagues (with whom they have published in the last four years, with whom they have received joint funding in the last four years, or with whom they are currently writing a manuscript, or submitting a grant proposal), or by family members, friends, or anyone for whom bias might affect the nature of the review - see the code of conduct

Enrico Rezendee.g. John Doe [john@doe.com]

2023-10-02 14:13:02

Pedro Simões