Genomic relationships among diploid and polyploid species of the genus *Ludwigia* L. section *Jussiaea* using a combination of molecular cytogenetic, morphological, and crossing investigationsuse asterix (*) to get italics

D. Barloy, L. Portillo - Lemus, S. A. Krueger-Hadfield, V. Huteau, O. CoritonPlease use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"

2023

<p>ABSTRACTThe genus Ludwigia L. sectionJussiaeais composed of a polyploid species complex with 2x, 4x, 6x and 10x ploidy levels, suggesting possible hybrid origins. The aim of the present study is to understand the genomic relationships among diploid and polyploid species in the section Jussiaea. Morphological and cytogenetic observations, controlled crosses, genomicin situhybridization (GISH), and flow cytometry were used to characterize species, ploidy levels, ploidy patterns, and genomic composition across taxa. Genome sizes obtained were in agreement with the diploid, tetraploid, hexaploid, and decaploid ploidy levels. Results of GISH showed that progenitors ofLudwigia stolonifera(4x) wereLudwigia peploidessubsp.montevidensis(2x) andLudwigia helminthorrhiza(2x), which also participated for one part (2x) to theLudwigia ascendensgenome (4x).Ludwigia grandiflorasubsp.hexapetala(10x) resulted from the hybridization betweenL. stolonifera(4x) andLudwigia grandiflorasubsp.grandiflora(6x). One progenitor ofL. grandiflorasubsp.grandiflorawas identified asL. peploides(2x). Our results suggest the existence of several processes of hybridization, leading to polyploidy, and possibly allopolyploidy, in the section Jussiaea due to the diversity of ploidy levels. The success of GISH opens up the potential for future studies to identify other missing progenitors inLudwigiaL. as well as other taxa.</p>

GISH, invasive plant, Ludwigia L., Onagraceae, polyploidy, phylogenetics

NonePlease indicate the methods that may require specialised expertise during the peer review process (use a comma to separate various required expertises).

Hybridization / Introgression, Phylogenetics / Phylogenomics

Malika Ainouche (mailla.ainouche@univ-rennes1.fr) (person who accepted to be recommender), Andrew LEITCH (a.r.leitch@qmul.ac.uk), Christian PARISOD (christian.parisod@ips.unibe.ch), Jesús M. CASTILLO (manucas@us.es), Andreas Hussner (hussner@igb-berlin.de )

e.g. John Doe john@doe.com

No need for them to be recommenders of PCIEvolBiol. Please do not suggest reviewers for whom there might be a conflict of interest. Reviewers are not allowed to review preprints written by close colleagues (with whom they have published in the last four years, with whom they have received joint funding in the last four years, or with whom they are currently writing a manuscript, or submitting a grant proposal), or by family members, friends, or anyone for whom bias might affect the nature of the review - see the code of conduct

2023-01-11 13:47:18

Malika AINOUCHE