Latest recommendations
Id | Title | Authors | Abstract▲ | Picture | Thematic fields | Recommender | Reviewers | Submission date | |
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27 Jul 2020
Evolution of the DAN gene family in vertebratesJuan C. Opazo, Federico G. Hoffmann, Kattina Zavala, Scott V. Edwards https://doi.org/10.1101/794404An evolutionary view of a biomedically important gene familyRecommended by Kateryna Makova based on reviews by 2 anonymous reviewersThis manuscript [1] investigates the evolutionary history of the DAN gene family—a group of genes important for embryonic development of limbs, kidneys, and left-right axis speciation. This gene family has also been implicated in a number of diseases, including cancer and nephropathies. DAN genes have been associated with the inhibition of the bone morphogenetic protein (BMP) signaling pathway. Despite this detailed biochemical and functional knowledge and clear importance for development and disease, evolution of this gene family has remained understudied. The diversification of this gene family was investigated in all major groups of vertebrates. The monophyly of the gene members belonging to this gene family was confirmed. A total of five clades were delineated, and two novel lineages were discovered. The first lineage was only retained in cephalochordates (amphioxus), whereas the second one (GREM3) was retained by cartilaginous fish, holostean fish, and coelanth. Moreover, the patterns of chromosomal synteny in the chromosomal regions harboring DAN genes were investigated. Additionally, the authors reconstructed the ancestral gene repertoires and studied the differential retention/loss of individual gene members across the phylogeny. They concluded that the ancestor of gnathostome vertebrates possessed eight DAN genes that underwent differential retention during the evolutionary history of this group. During radiation of vertebrates, GREM1, GREM2, SOST, SOSTDC1, and NBL1 were retained in all major vertebrate groups. At the same time, GREM3, CER1, and DAND5 were differentially lost in some vertebrate lineages. At least two DAN genes were present in the common ancestor of vertebrates, and at least three DAN genes were present in the common ancestor of chordates. Therefore the patterns of retention and diversification in this gene family appear to be complex. Evolutionary slowdown for the DAN gene family was observed in mammals, suggesting selective constraints. Overall, this article puts the biomedical importance of the DAN family in the evolutionary perspective. References [1] Opazo JC, Hoffmann FG, Zavala K, Edwards SV (2020) Evolution of the DAN gene family in vertebrates. bioRxiv, 794404, ver. 3 peer-reviewed and recommended by PCI Evolutionary Biology. doi: 10.1101/794404 | Evolution of the DAN gene family in vertebrates | Juan C. Opazo, Federico G. Hoffmann, Kattina Zavala, Scott V. Edwards | <p>The DAN gene family (DAN, Differential screening-selected gene Aberrant in Neuroblastoma) is a group of genes that is expressed during development and plays fundamental roles in limb bud formation and digitation, kidney formation and morphogene... | Molecular Evolution | Kateryna Makova | 2019-10-15 16:43:13 | View | ||
16 May 2023
A new and almost perfectly accurate approximation of the eigenvalue effective population size of a dioecious population: comparisons with other estimates and detailed proofsThierry de Meeûs and Camille Noûs https://doi.org/10.5281/zenodo.7927968All you ever wanted to know about Ne in one handy placeRecommended by Charles Baer based on reviews by Jesse ("Jay") Taylor and 1 anonymous reviewerOf the four evolutionary forces, three can be straightforwardly summarized both conceptually and mathematically in the context of an allele at a genomic locus. Mutation (the mutation rate, μ) is simply captured by the per-site, per-generation probability that an allele mutates into a different allele. Recombination (the recombination rate, r) is captured as the probability of recombination between two sites, wherein alleles that are in different genomes in one generation come together in the same genome in the next generation. Natural selection (the selection coefficient, s) is captured by the probability that an allele is present in the next generation, relative to some reference. Random genetic drift – the random fluctuation in allele frequency due to sampling in a finite population - is not so straightforwardly summarized. The first, and most common way of characterizing evolutionary dynamics in a finite population is the Wright-Fisher model, in which the only deviation from the assumptions of Hardy-Weinberg conditions is finite population size. Importantly, in a W-F population, mating between diploid individuals is random, which implies self-fertile monoecy, and generations are non-overlapping. In an ideal W-F population, the probability that a gene copy leaves i descendants in the next generation is the result of binomial sampling of uniting gametes (if the locus is biallelic). The – and the next word is meaningful – magnitude/strength/rate/power/amount of genetic drift is proportional to 1/2N, where N is the size of the population. All of the following are affected by genetic drift: (1) the probability that a neutral allele ultimately reaches fixation, (2) the rate of loss of genetic variation within a population, (3) the rate of increase of genetic variance among populations, (4) the amount of genetic variation segregating in a population, (5) the probability of fixation/loss of a weakly selected variant. Presumably no real population adheres to ideal W-F conditions, which leads to the notion of "effective population size", Ne (Wright 1931), loosely defined as "the size of an ideal W-F population that experiences an equivalent strength of genetic drift". Almost always, Ne<N, and any violation of W-F assumptions can affect Ne. Importantly, Ne can be defined in different ways, and the specific formulation of Ne can have different implications for evolution. Ne was initially defined in terms of the rate of decrease of heterozygosity (inbreeding effective size) and increase in variance among populations (variance effective size). Ewens (1979) defined the Eigenvalue effective size (equivalent to the "random extinction" effective size) and elaborated on the conditions under which the various formulations of Ne differ (Ewens 1982). Nordborg and Krone (2002) defined the effective size in terms of the coalescent, and they identified conditions in which genetic drift cannot be described in terms of a W-F model (Sjodin et al. 2005); also see Karasov et al. (2010); Neher and Shraiman (2011). Distinct from the issue of defining Ne is the issue of calculating Ne from data, which is the focus of this paper by De Meeus and Noûs (2023). Pudovkin et al. (1996) showed that the Eigenvalue effective size in a dioecious population can be formulated in terms of excess heterozygosity, which the current authors note is equivalent to formulating Ne in terms of Wright's FIS statistic. As emphasized by the title, the marquee contribution of this paper is to provide a better approximation of the Eigenvalue effective size in a dioecious population. Science marches onward, although the empirical utility of this advance is obviously limited, given the tremendous inherent sources of uncertainty in real-world estimates of Ne. Perhaps more valuable, however, is the extensive set of appendixes, in which detailed derivations are provided for the various formulations of effective size. By way of analogy, the material presented here can be thought of as an extension of the material presented in section 7.6 of Crow and Kimura (1970), in which the Inbreeding and Variance effective population sizes are derived and compared. The appendixes should serve as a handy go-to source of detailed theoretical information with respect to the different formulations of effective population size. REFERENCES Crow, J. F. and M. Kimura. 1970. An Introduction to Population Genetics Theory. The Blackburn Press, Caldwell, NJ. De Meeûs, T. and Noûs, C. 2023. A new and almost perfectly accurate approximation of the eigenvalue effective population size of a dioecious population: comparisons with other estimates and detailed proofs. Zenodo, ver. 6 peer-reviewed and recommended by Peer Community in Evolutionary Biology. https://doi.org/10.5281/zenodo.7927968 Ewens, W. J. 1979. Mathematical Population Genetics. Springer-Verlag, Berlin. Ewens, W. J. 1982. On the concept of the effective population size. Theoretical Population Biology 21:373-378. https://doi.org/10.1016/0040-5809(82)90024-7 Karasov, T., P. W. Messer, and D. A. Petrov. 2010. Evidence that adaptation in Drosophila Is not limited by mutation at single sites. Plos Genetics 6. https://doi.org/10.1371/journal.pgen.1000924 Neher, R. A. and B. I. Shraiman. 2011. Genetic Draft and Quasi-Neutrality in Large Facultatively Sexual Populations. Genetics 188:975-U370. https://doi.org/10.1534/genetics.111.128876 Nordborg, M. and S. M. Krone. 2002. Separation of time scales and convergence to the coalescent in structured populations. Pp. 194–232 in M. Slatkin, and M. Veuille, eds. Modern Developments in Theoretical Population Genetics: The Legacy of Gustave Malécot. Oxford University Press, Oxford. https://www.webpages.uidaho.edu/~krone/malecot.pdf Pudovkin, A. I., D. V. Zaykin, and D. Hedgecock. 1996. On the potential for estimating the effective number of breeders from heterozygote-excess in progeny. Genetics 144:383-387. https://doi.org/10.1093/genetics/144.1.383 Sjodin, P., I. Kaj, S. Krone, M. Lascoux, and M. Nordborg. 2005. On the meaning and existence of an effective population size. Genetics 169:1061-1070. https://doi.org/10.1534/genetics.104.026799 Wright, S. 1931. Evolution in Mendelian populations. Genetics 16:0097-0159. https://doi.org/10.1093/genetics/16.2.97 | A new and almost perfectly accurate approximation of the eigenvalue effective population size of a dioecious population: comparisons with other estimates and detailed proofs | Thierry de Meeûs and Camille Noûs | <p>The effective population size is an important concept in population genetics. It corresponds to a measure of the speed at which genetic drift affects a given population. Moreover, this is most of the time the only kind of population size that e... | Bioinformatics & Computational Biology, Evolutionary Ecology, Evolutionary Theory, Population Genetics / Genomics, Reproduction and Sex | Charles Baer | 2023-02-22 16:53:49 | View | ||
05 May 2020
Meta-population structure and the evolutionary transition to multicellularityCaroline J Rose, Katrin Hammerschmidt, Yuriy Pichugin and Paul B Rainey https://doi.org/10.1101/407163The ecology of evolutionary transitions to multicellularityRecommended by Dustin Brisson based on reviews by 2 anonymous reviewersThe evolutionary transition to multicellular life from free-living, single-celled ancestors has occurred independently in multiple lineages [1-5]. This evolutionary transition to cooperative group living can be difficult to explain given the fitness advantages enjoyed by the non-cooperative, single-celled organisms that still numerically dominate life on earth [1,6,7]. Although several hypotheses have been proposed to explain the transition to multicellularity, a common theme is the abatement of the efficacy of natural selection among the single cells during the free-living stage and the promotion of the efficacy of selection among groups of cells during the cooperative stage, an argument reminiscent of those from George Williams’ seminal book [8,9]. The evolution of life cycles appears to be a key step in the transition to multicellularity as it can align fitness advantages of the single-celled 'reproductive' stage with that of the cooperative 'organismal' stage [9-12]. That is, the evolution of life cycles allows natural selection to operate over timescales longer than that of the doubling time of the free-living cells [13]. Despite the importance of this issue, identifying the range of ecological conditions that reduce the importance of natural selection at the single-celled, free-living stage and increase the importance of selection among groups of cooperating cells has not been addressed empirically. References [1] Maynard Smith, J. and Szathmáry, E. (1995). The Major Transitions in Evolution. Oxford, UK: Freeman. p 346. | Meta-population structure and the evolutionary transition to multicellularity | Caroline J Rose, Katrin Hammerschmidt, Yuriy Pichugin and Paul B Rainey | <p>The evolutionary transition to multicellularity has occurred on numerous occasions, but transitions to complex life forms are rare. While the reasons are unclear, relevant factors include the intensity of within- versus between-group selection ... | Adaptation, Evolutionary Dynamics, Experimental Evolution | Dustin Brisson | 2019-04-04 12:26:36 | View | ||
09 Dec 2019
Trait-specific trade-offs prevent niche expansion in two parasitesEva JP Lievens, Yannis Michalakis, Thomas Lenormand https://doi.org/10.1101/621581Trade-offs in fitness components and ecological source-sink dynamics affect host specialisation in two parasites of Artemia shrimpsRecommended by Frédéric Guillaume based on reviews by Anne Duplouy, Seth Barribeau and Cindy GidoinEcological specialisation, especially among parasites infecting a set of host species, is ubiquitous in nature. Host specialisation can be understood as resulting from trade-offs in parasite infectivity, virulence and growth. However, it is not well understood how variation in these trade-offs shapes the overall fitness trade-off a parasite faces when adapting to multiple hosts. For instance, it is not clear whether a strong trade-off in one fitness component may sufficiently constrain the evolution of a generalist parasite despite weak trade-offs in other components. A second mechanism explaining variation in specialisation among species is habitat availability and quality. Rare habitats or habitats that act as ecological sinks will not allow a species to persist and adapt, preventing a generalist phenotype to evolve. Understanding the prevalence of those mechanisms in natural systems is crucial to understand the emergence and maintenance of host specialisation, and biodiversity in general. References [1] Lievens, E.J.P., Michalakis, Y. and Lenormand, T. (2019). Trait-specific trade-offs prevent niche expansion in two parasites. bioRxiv, 621581, ver. 4 peer-reviewed and recommended by PCI Evolutionary Biology. doi: 10.1101/621581 | Trait-specific trade-offs prevent niche expansion in two parasites | Eva JP Lievens, Yannis Michalakis, Thomas Lenormand | <p>The evolution of host specialization has been studied intensively, yet it is still often difficult to determine why parasites do not evolve broader niches – in particular when the available hosts are closely related and ecologically similar. He... | Adaptation, Evolutionary Ecology, Evolutionary Epidemiology, Experimental Evolution, Life History, Species interactions | Frédéric Guillaume | 2019-05-13 13:44:34 | View | ||
28 Sep 2020
Evolution and genetic architecture of disassortative mating at a locus under heterozygote advantageLudovic Maisonneuve, Mathieu Joron, Mathieu Chouteau and Violaine Llaurens https://doi.org/10.1101/616409Evolutionary insights into disassortative mating and its association to an ecologically relevant supergeneRecommended by Charles Mullon based on reviews by Tom Van Dooren and 2 anonymous reviewersHeliconius butterflies are famous for their colorful wing patterns acting as a warning of their chemical defenses [1]. Most species are involved in Müllerian mimicry assemblies, as predators learn to associate common wing patterns with unpalatability and preferentially target rare variants. Such positive-frequency dependent selection homogenizes wing patterns at different localities, and in several species, all individuals within a community belong to the same morph [2]. In this respect, H. numata stands out. This species shows stable local polymorphism across multiple localities, with local populations home to up to seven distinct morphs [2]. Although a balance between migration and local positive-frequency dependent selection can allow some degree of local polymorphism, theory suggests that this occurs only when migration is within a narrow window [3]. References [1] Merrill, R M, K K Dasmahapatra, J W Davey, D D Dell'Aglio, J J Hanly, B Huber, C D Jiggins, et al. (2015). The Diversification of Heliconius butterflies: What Have We Learned in 150 Years? Journal of Evolutionary Biology 28 (8), 1417–38. https://doi.org/10.1111/jeb.12672. | Evolution and genetic architecture of disassortative mating at a locus under heterozygote advantage | Ludovic Maisonneuve, Mathieu Joron, Mathieu Chouteau and Violaine Llaurens | <p>The evolution of mate preferences may depend on natural selection acting on the mating cues and on the underlying genetic architecture. While the evolution of assortative mating with respect to locally adapted traits has been well-characterized... | Evolutionary Theory, Population Genetics / Genomics, Reproduction and Sex, Sexual Selection | Charles Mullon | 2019-10-29 09:55:18 | View | ||
11 Jul 2022
Mutualists construct the ecological conditions that trigger the transition from parasitismLeo Ledru, Jimmy Garnier, Matthias Rohr, Camille Nous, Sebastien Ibanez https://www.biorxiv.org/content/10.1101/2021.08.18.456759Give them some space: how spatial structure affects the evolutionary transition towards mutualistic symbiosisRecommended by Francois Massol based on reviews by Eva Kisdi and 3 anonymous reviewersThe evolution of mutualistic symbiosis is a puzzle that has fascinated evolutionary ecologist for quite a while. Data on transitions between symbiotic bacterial ways of life has evidenced shifts from mutualism towards parasitism and vice versa (Sachs et al., 2011), so there does not seem to be a strong determinism on those transitions. From the host’s perspective, mutualistic symbiosis implies at the very least some form of immune tolerance, which can be costly (e.g. Sorci, 2013). Empirical approaches thus raise very important questions: How can symbiosis turn from parasitism into mutualism when it seemingly needs such a strong alignment of selective pressures on both the host and the symbiont? And yet why is mutualistic symbiosis so widespread and so important to the evolution of macro-organisms (Margulis, 1998)? While much of the theoretical literature on the evolution of symbiosis and mutualism has focused on either the stability of such relationships when non-mutualists can invade the host-symbiont system (e.g. Ferrière et al., 2007) or the effect of the mode of symbiont transmission on the evolutionary dynamics of mutualism (e.g. Genkai-Kato and Yamamura, 1999), the question remains whether and under which conditions parasitic symbiosis can turn into mutualism in the first place. Earlier results suggested that spatial demographic heterogeneity between host populations could be the leading determinant of evolution towards mutualism or parasitism (Hochberg et al., 2000). Here, Ledru et al. (2022) investigate this question in an innovative way by simulating host-symbiont evolutionary dynamics in a spatially explicit context. Their hypothesis is intuitive but its plausibility is difficult to gauge without a model: Does the evolution towards mutualism depend on the ability of the host and symbiont to evolve towards close-range dispersal in order to maintain clusters of efficient host-symbiont associations, thus outcompeting non-mutualists? I strongly recommend reading this paper as the results obtained by the authors are very clear: competition strength and the cost of dispersal both affect the likelihood of the transition from parasitism to mutualism, and once mutualism has set in, symbiont trait values clearly segregate between highly dispersive parasites and philopatric mutualists. The demonstration of the plausibility of their hypothesis is accomplished with brio and thoroughness as the authors also examine the conditions under which the transition can be reversed, the impact of the spatial range of competition and the effect of mortality. Since high dispersal cost and strong, long-range competition appear to be the main factors driving the evolutionary transition towards mutualistic symbiosis, now is the time for empiricists to start investigating this question with spatial structure in mind. References Ferrière, R., Gauduchon, M. and Bronstein, J. L. (2007) Evolution and persistence of obligate mutualists and exploiters: competition for partners and evolutionary immunization. Ecology Letters, 10, 115-126. https://doi.org/10.1111/j.1461-0248.2006.01008.x Genkai-Kato, M. and Yamamura, N. (1999) Evolution of mutualistic symbiosis without vertical transmission. Theoretical Population Biology, 55, 309-323. https://doi.org/10.1006/tpbi.1998.1407 Hochberg, M. E., Gomulkiewicz, R., Holt, R. D. and Thompson, J. N. (2000) Weak sinks could cradle mutualistic symbioses - strong sources should harbour parasitic symbioses. Journal of Evolutionary Biology, 13, 213-222. https://doi.org/10.1046/j.1420-9101.2000.00157.x Ledru L, Garnier J, Rohr M, Noûs C and Ibanez S (2022) Mutualists construct the ecological conditions that trigger the transition from parasitism. bioRxiv, 2021.08.18.456759, ver. 5 peer-reviewed and recommended by Peer Community in Evolutionary Biology. https://doi.org/10.1101/2021.08.18.456759 Margulis, L. (1998) Symbiotic planet: a new look at evolution, Basic Books, Amherst. Sachs, J. L., Skophammer, R. G. and Regus, J. U. (2011) Evolutionary transitions in bacterial symbiosis. Proceedings of the National Academy of Sciences, 108, 10800-10807. https://doi.org/10.1073/pnas.1100304108 Sorci, G. (2013) Immunity, resistance and tolerance in bird–parasite interactions. Parasite Immunology, 35, 350-361. https://doi.org/10.1111/pim.12047 | Mutualists construct the ecological conditions that trigger the transition from parasitism | Leo Ledru, Jimmy Garnier, Matthias Rohr, Camille Nous, Sebastien Ibanez | <p>The evolution of mutualism between hosts and initially parasitic symbionts represents a major transition in evolution. Although vertical transmission of symbionts during host reproduction and partner control both favour the stability of mutuali... | Evolutionary Ecology, Species interactions | Francois Massol | 2021-08-20 12:25:40 | View | ||
04 Jul 2022
A genomic assessment of the marine-speciation paradox within the toothed whale superfamily DelphinoideaMichael V Westbury, Andrea A Cabrera, Alba Rey-Iglesia, Binia De Cahsan, David A. Duchêne, Stefanie Hartmann, Eline D Lorenzen https://doi.org/10.1101/2020.10.23.352286Reticulated evolution marks the rapid diversification of the DelphinoidaeRecommended by Michael C. Fontaine based on reviews by Christelle Fraïsse, Simon Henry Martin, Andrew Foote and 2 anonymous reviewersHistorically neglected or considered a rare aberration in animals under the biological species concept, interspecific hybridisation has by now been recognised to be taxonomically widespread, particularly in rapidly diversifying groups (Dagilis et al. 2021; Edelman & Mallet 2021; Mallet et al. 2016; Seehausen 2004). Yet the prevalence of introgressive hybridizations, its evolutionary significance, and its impact on species diversification remain a hot topic of research in evolutionary biology. The rapid increase in genomic resources now available for non-model species has significantly contributed to the detection of introgressive hybridization across taxa showing that reticulated evolution is far more common in the animal kingdom than historically considered. Yet, detecting it, quantifying its magnitude, and assessing its evolutionary significance remains a challenging endeavour with constantly evolving methodologies to better explore and exploit genomic data (Blair & Ané 2020; Degnan & Rosenberg 2009; Edelman & Mallet 2021; Hibbins & Hahn 2022). In the marine realm, the dearth of geographic barriers and the large dispersal abilities of pelagic species like cetaceans have raised the questions of how populations and species can diverge and adapt to distinct ecological conditions in face of potentially large gene-flow, the so-called marine speciation paradox (Bierne et al. 2003). Contemporaneous hybridization among cetacean species has been widely documented in nature despite large phenotypic differences (Crossman et al. 2016). The historical prevalence of reticulated evolution, its evolutionary significance, and how it might have impacted the evolutionary history and diversification of the cetaceans have however remained elusive so far. Recent phylogenomic studies suggested that introgression has been prevalent in cetacean evolutionary history with instances reported among baleen whales (mysticetes) (Árnason et al. 2018) and among toothed whales (odontocetes), especially in the rapidly diversifying dolphins family of the Delphininae (Guo et al. 2021; Moura et al. 2020). Analysing publicly available whole-genome data from nine cetacean species across three families of Delphinoidae – dolphins, porpoises, and monondontidae – using phylogenomics and demo-genetics approaches, Westbury and colleagues (2022) take a step further in documenting that evolution among these species has been far from a simple bifurcating tree. Instead, their study describes widespread occurrences of introgression among Delphinoidae, drawing a complex picture of reticulated evolutionary history. After describing major topology discordance in phylogenetic gene trees along the genome, the authors use a panel of approaches to disentangle introgression from incomplete lineage sorting (ILS), the two most common causes of tree topology discordances (Hibbins & Hahn 2022). Applying popular tests that separate introgression from ILS, such as the Patterson’s D (a.k.a. ABBA-BABA) test (Durand et al. 2011; Green et al. 2010), QuIBL (Edelman et al. 2019), and D-FOIL (Pease & Hahn 2015), the authors report that signals of introgression are present in the genomes of most (if not all) the cetacean species included in their study. However, this picture needs to be nuanced. Most introgression signals seem to echo old introgression events that occurred primarily among ancestors. This is suggested by the differential signals of topology discordance along the genome when considering sliding windows along the genome of varying sizes (50kb, 100kb, and 1Mb), and by patterns of excess derived allele sharing along branches of the species tree, as captured by the f-branch test (Malinsky et al. 2021; Malinsky et al. 2018). The authors further investigated the dynamic of cessation of gene flow (and/or ILS) between species using the F1 hybrid PSMC (or hPSMC) approach (Cahill et al. 2016). By estimating the cross-coalescent rates (CRR) between species pairs with time in light of previously estimated species divergence times (McGowen et al. 2020), the authors report that gene flow (and/or ILS) most likely has stopped by now among most species, but it may have lasted for more than half of the time since the species split from each other. According to the author, this result may reflect the slow process by which reproductive isolation would have evolved between cetacean lineages, and that species isolation was marked by significant introgression events. Now, while the present study provides a good overview of how complex is the reticulated evolutionary history of the Delphinoidae, getting a complete picture will require overcoming a few important limitations. The first ones are methodological and related to the phylogenomic analyses. Given the sampling design with one diploid genome per species, the authors could not phase the data into the parental haplotypes, but instead relied on a majority consensus creating mosaic haploidized genomes representing a mixture between the two parental copies. Moreover, by using large genomic windows (≥50kb) that likely span multiple independent loci, phylogenetic analyses in windows encompassed distinct phylogenetic signals, potentially leading to bias and inaccuracy in the inferences. Thawornwattana et al (2018) previously showed that this “concatenation approach” could significantly impact phylogenetic inferences. They proposed instead to use loci small enough to minimise the risk of intra-locus recombination and to consider them in blocks of non-recombining loci along the genome in order to conduct phylogenetic analysed, ideally under the multi-species coalescent (MSC) that can account for ILS (e.g. BPP; Flouri et al. 2018; Jiao et al. 2020; Yang 2015). Such an approach applied to the diversification of the Delphinidae may reveal substantial changes compared to the currently admitted species tree. Inaccuracy in the species tree estimation may have a major impact on the introgression analyses conducted in this study since the species tree and branching order must be supplied in the introgression analyses to properly disentangle introgression from ILS. Here, the authors rely on the tree topology that was previously estimated in McGowen et al. (2020), which they also recovered using their consensus estimation from ASTRAL-III (Zhang et al. 2018). While the methodologies accounted to a certain extent for ILS, albeit with potential bias induced by the concatenation approach, they ignore the presumably large amount of introgression among species during the diversification process. Estimating species branching order while ignoring introgression can lead to major bias in the phylogenetic inference and can lead to incorrect topologies. Even if these MSC-based methods account for ILS, inferences can become very inaccurate or even break down as gene flow increases (see for ex. Jiao et al. 2020; Müller et al. in press; Solís-Lemus et al. 2016). Dedicated approaches have been developed to model explicitly introgression together with ILS to estimate phylogenetic networks (Blair & Ané 2020; Rabier et al. 2021) or in isolation-with-migration model (Müller et al. in press; Wang et al. 2020). Future studies revisiting the reticulated evolutionary history of the Delphinoidae with such approaches may not only precise the species branching order, but also deliver a finer view of the magnitude and prevalence of introgression during the evolutionary history of these species. A final part of Westbury et al. (2022)'s study set out to test whether historical periods of low abundance could have facilitated hybridization among Delphinoidae species. During these periods of low abundance, species may encounter only a limited number of conspecifics and may consider individuals from other species as suitable mating partners, leading to hybridisation (Crossman et al. 2016; Edwards et al. 2011; Westbury et al. 2019). The authors tested this hypothesis by considering genome-wide genetic diversity (or heterozygosity) as a proxy of historical effective population size (Ne), itself as a proxy of the evolution of census size with time. They also try to link historical Ne variation (from PSMC, Li & Durbin 2011) with their estimated time to cessation of gene flow or ILS (from the CRR of hPSMC). However, no straightforward relationship was found between the genetic diversity and the propensity of species to hybridize, nor was there any clear link between Ne variation through time and the cessation of gene flow or ILS. Such a lack of relationship may not come as a surprise, since the determinants of genome-wide genetic diversity and its variation through evolutionary time-scale are far more diverse and complex than just a direct link with hybridization, introgression, or even with the census population size. In fact, genetic diversity results from the balance between all the evolutionary processes at play in the species' evolutionary history (see the review of Ellegren & Galtier 2016). Other important factors can strongly impact genetic diversity, including demography and structure, but also linked selection (Boitard et al. 2022; Buffalo 2021; Ellegren & Galtier 2016). All in all, Westbury and coll. (2022) present here an interesting study providing an additional step towards resolving and understanding the complex evolutionary history of the Delphinoidae, and shedding light on the importance of introgression during the diversification of these cetacean species. Prospective work improving upon the taxonomic sampling, with additional genomic data for each species, considered with dedicated approaches tailored at estimating species tree or network while accounting for ILS and introgression will be key for refining the picture depicted in this study. Down the road, altogether these studies will contribute to assessing the evolutionary significance of introgression on the diversification of Delphinoides, and more generally on the diversification of cetacean species, which still remain an open and exciting perspective. References Árnason Ú, Lammers F, Kumar V, Nilsson MA, Janke A (2018) Whole-genome sequencing of the blue whale and other rorquals finds signatures for introgressive gene flow. Science Advances 4, eaap9873. https://doi.org/10.1126/sciadv.aap9873 Bierne N, Bonhomme F, David P (2003) Habitat preference and the marine-speciation paradox. Proceedings of the Royal Society of London. Series B: Biological Sciences 270, 1399-1406. https://doi.org/10.1098/rspb.2003.2404 Blair C, Ané C (2020) Phylogenetic Trees and Networks Can Serve as Powerful and Complementary Approaches for Analysis of Genomic Data. Systematic Biology 69, 593-601. https://doi.org/10.1093/sysbio/syz056 Boitard S, Arredondo A, Chikhi L, Mazet O (2022) Heterogeneity in effective size across the genome: effects on the inverse instantaneous coalescence rate (IICR) and implications for demographic inference under linked selection. Genetics 220, iyac008. https://doi.org/10.1093/genetics/iyac008 Buffalo V (2021) Quantifying the relationship between genetic diversity and population size suggests natural selection cannot explain Lewontin's Paradox. e-Life 10, e67509. https://doi.org/10.7554/eLife.67509 Cahill JA, Soares AE, Green RE, Shapiro B (2016) Inferring species divergence times using pairwise sequential Markovian coalescent modelling and low-coverage genomic data. Philos Trans R Soc Lond B Biol Sci 371, 20150138. https://doi.org/10.1098/rstb.2015.0138 Crossman CA, Taylor EB, Barrett‐Lennard LG (2016) Hybridization in the Cetacea: widespread occurrence and associated morphological, behavioral, and ecological factors. Ecology and Evolution 6, 1293-1303. https://doi.org/10.1002/ece3.1913 Dagilis AJ, Peede D, Coughlan JM, Jofre GI, D’Agostino ERR, Mavengere H, Tate AD, Matute DR (2021) 15 years of introgression studies: quantifying gene flow across Eukaryotes. biorXiv, 2021.1106.1115.448399. https://doi.org/10.1101/2021.06.15.448399 Degnan JH, Rosenberg NA (2009) Gene tree discordance, phylogenetic inference and the multispecies coalescent. Trends Ecol Evol 24, 332-340. https://doi.org/10.1016/j.tree.2009.01.009 Durand EY, Patterson N, Reich D, Slatkin M (2011) Testing for ancient admixture between closely related populations. Mol Biol Evol 28, 2239-2252. https://doi.org/10.1093/molbev/msr048 Edelman NB, Frandsen PB, Miyagi M, Clavijo B, Davey J, Dikow RB, Garcia-Accinelli G, Van Belleghem SM, Patterson N, Neafsey DE, Challis R, Kumar S, Moreira GRP, Salazar C, Chouteau M, Counterman BA, Papa R, Blaxter M, Reed RD, Dasmahapatra KK, Kronforst M, Joron M, Jiggins CD, McMillan WO, Di Palma F, Blumberg AJ, Wakeley J, Jaffe D, Mallet J (2019) Genomic architecture and introgression shape a butterfly radiation. Science 366, 594-599. https://doi.org/10.1126/science.aaw2090 Edelman NB, Mallet J (2021) Prevalence and Adaptive Impact of Introgression. Annual Review of Genetics 55, 265-283. https://doi.org/10.1146/annurev-genet-021821-020805 Edwards CJ, Suchard MA, Lemey P, Welch JJ, Barnes I, Fulton TL, Barnett R, O'Connell TC, Coxon P, Monaghan N, Valdiosera CE, Lorenzen ED, Willerslev E, Baryshnikov GF, Rambaut A, Thomas MG, Bradley DG, Shapiro B (2011) Ancient hybridization and an Irish origin for the modern polar bear matriline. Curr Biol 21, 1251-1258. https://doi.org/10.1016/j.cub.2011.05.058 Ellegren H, Galtier N (2016) Determinants of genetic diversity. Nat Rev Genet 17, 422-433. https://doi.org/10.1038/nrg.2016.58 Flouri T, Jiao X, Rannala B, Yang Z (2018) Species Tree Inference with BPP Using Genomic Sequences and the Multispecies Coalescent. Mol Biol Evol 35, 2585-2593. https://doi.org/10.1093/molbev/msy147 Green RE, Krause J, Briggs AW, Maricic T, Stenzel U, Kircher M, Patterson N, Li H, Zhai W, Fritz MH, Hansen NF, Durand EY, Malaspinas AS, Jensen JD, Marques-Bonet T, Alkan C, Prufer K, Meyer M, Burbano HA, Good JM, Schultz R, Aximu-Petri A, Butthof A, Hober B, Hoffner B, Siegemund M, Weihmann A, Nusbaum C, Lander ES, Russ C, Novod N, Affourtit J, Egholm M, Verna C, Rudan P, Brajkovic D, Kucan Z, Gusic I, Doronichev VB, Golovanova LV, Lalueza-Fox C, de la Rasilla M, Fortea J, Rosas A, Schmitz RW, Johnson PLF, Eichler EE, Falush D, Birney E, Mullikin JC, Slatkin M, Nielsen R, Kelso J, Lachmann M, Reich D, Paabo S (2010) A draft sequence of the Neandertal genome. Science 328, 710-722. https://doi.org/10.1126/science.1188021 Guo W, Sun D, Cao Y, Xiao L, Huang X, Ren W, Xu S, Yang G (2021) Extensive Interspecific Gene Flow Shaped Complex Evolutionary History and Underestimated Species Diversity in Rapidly Radiated Dolphins. Journal of Mammalian Evolution 29, 353-367. https://doi.org/10.1007/s10914-021-09581-6 Hibbins MS, Hahn MW (2022) Phylogenomic approaches to detecting and characterizing introgression. Genetics 220, iyab173. https://doi.org/10.1093/genetics/iyab173 Jiao X, Flouri T, Rannala B, Yang Z (2020) The Impact of Cross-Species Gene Flow on Species Tree Estimation. Syst Biol 69, 830-847. https://doi.org/10.1093/sysbio/syaa001 Li H, Durbin R (2011) Inference of human population history from individual whole-genome sequences. Nature 475, 493-496. https://doi.org/10.1038/nature10231 Malinsky M, Matschiner M, Svardal H (2021) Dsuite - Fast D-statistics and related admixture evidence from VCF files. Mol Ecol Resour 21, 584-595. https://doi.org/10.1111/1755-0998.13265 Malinsky M, Svardal H, Tyers AM, Miska EA, Genner MJ, Turner GF, Durbin R (2018) Whole-genome sequences of Malawi cichlids reveal multiple radiations interconnected by gene flow. Nature Ecology & Evolution 2, 1940-1955. https://doi.org/10.1038/s41559-018-0717-x Mallet J, Besansky N, Hahn MW (2016) How reticulated are species? Bioessays 38, 140-149. https://doi.org/10.1002/bies.201500149 McGowen MR, Tsagkogeorga G, Alvarez-Carretero S, Dos Reis M, Struebig M, Deaville R, Jepson PD, Jarman S, Polanowski A, Morin PA, Rossiter SJ (2020) Phylogenomic Resolution of the Cetacean Tree of Life Using Target Sequence Capture. Syst Biol 69, 479-501. https://doi.org/10.1093/sysbio/syz068 Moura AE, Shreves K, Pilot M, Andrews KR, Moore DM, Kishida T, Möller L, Natoli A, Gaspari S, McGowen M, Chen I, Gray H, Gore M, Culloch RM, Kiani MS, Willson MS, Bulushi A, Collins T, Baldwin R, Willson A, Minton G, Ponnampalam L, Hoelzel AR (2020) Phylogenomics of the genus Tursiops and closely related Delphininae reveals extensive reticulation among lineages and provides inference about eco-evolutionary drivers. Molecular Phylogenetics and Evolution 146,107047. https://doi.org/10.1016/j.ympev.2020.106756 Müller NF, Ogilvie HA, Zhang C, Fontaine MC, Amaya-Romero JE, Drummond AJ, Stadler T (in press) Joint inference of species histories and gene flow. Syst Biol. Pease JB, Hahn MW (2015) Detection and Polarization of Introgression in a Five-Taxon Phylogeny. Syst Biol 64, 651-662. https://doi.org/10.1093/sysbio/syv023 Rabier CE, Berry V, Stoltz M, Santos JD, Wang W, Glaszmann JC, Pardi F, Scornavacca C (2021) On the inference of complex phylogenetic networks by Markov Chain Monte-Carlo. PLoS Comput Biol 17, e1008380. https://doi.org/10.1371/journal.pcbi.1008380 Seehausen O (2004) Hybridization and adaptive radiation. Trends Ecol Evol 19, 198-207. https://doi.org/10.1016/j.tree.2004.01.003 Solís-Lemus C, Yang M, Ané C (2016) Inconsistency of Species Tree Methods under Gene Flow. Syst Biol 65, 843-851. https://doi.org/10.1093/sysbio/syw030 Thawornwattana Y, Dalquen D, Yang Z, Tamura K (2018) Coalescent Analysis of Phylogenomic Data Confidently Resolves the Species Relationships in the Anopheles gambiae Species Complex. Molecular Biology and Evolution 35, 2512-2527. https://doi.org/10.1093/molbev/msy158 Wang K, Mathieson I, O’Connell J, Schiffels S (2020) Tracking human population structure through time from whole genome sequences. PLOS Genetics 16, e1008552. https://doi.org/10.1371/journal.pgen.1008552 Westbury MV, Cabrera AA, Rey-Iglesia A, Cahsan BD, Duchêne DA, Hartmann S, Lorenzen ED (2022) A genomic assessment of the marine-speciation paradox within the toothed whale superfamily Delphinoidea. bioRxiv, 2020.10.23.352286, ver. 7 peer-reviewed and recommended by Peer Community in Evolutionary Biology. https://doi.org/10.1101/2020.10.23.352286 Westbury MV, Petersen B, Lorenzen ED (2019) Genomic analyses reveal an absence of contemporary introgressive admixture between fin whales and blue whales, despite known hybrids. PLoS ONE 14, e0222004. https://doi.org/10.1371/journal.pone.0222004 Yang Z (2015) The BPP program for species tree estimation and species delimitation. Current Zoology 61, 854-865. https://doi.org/10.1093/czoolo/61.5.854 Zhang C, Rabiee M, Sayyari E, Mirarab S (2018) ASTRAL-III: polynomial time species tree reconstruction from partially resolved gene trees. BMC Bioinformatics 19, 153. https://doi.org/10.1186/s12859-018-2129-y | A genomic assessment of the marine-speciation paradox within the toothed whale superfamily Delphinoidea | Michael V Westbury, Andrea A Cabrera, Alba Rey-Iglesia, Binia De Cahsan, David A. Duchêne, Stefanie Hartmann, Eline D Lorenzen | <p>The importance of post-divergence gene flow in speciation has been documented across a range of taxa in recent years, and may have been especially widespread in highly mobile, wide-ranging marine species, such as cetaceans. Here, we studied ind... | Evolutionary Dynamics, Hybridization / Introgression, Molecular Evolution, Phylogenetics / Phylogenomics, Speciation | Michael C. Fontaine | 2020-10-25 08:55:50 | View | ||
25 Feb 2021
Alteration of gut microbiota with a broad-spectrum antibiotic does not impair maternal care in the European earwigSophie Van Meyel, Séverine Devers, Simon Dupont, Franck Dedeine and Joël Meunier https://doi.org/10.1101/2020.10.08.331363Assessing the role of host-symbiont interactions in maternal care behaviourRecommended by Trine Bilde based on reviews by Nadia Aubin-Horth, Gabrielle Davidson and 1 anonymous reviewerThe role of microbial symbionts in governing social traits of their hosts is an exciting and developing research area. Just like symbionts influence host reproductive behaviour and can cause mating incompatibilities to promote symbiont transmission through host populations (Engelstadter and Hurst 2009; Correa and Ballard 2016; Johnson and Foster 2018) (see also discussion on conflict resolution in Johnsen and Foster 2018), microbial symbionts could enhance transmission by promoting the social behaviour of their hosts (Ezenwa et al. 2012; Lewin-Epstein et al. 2017; Gurevich et al. 2020). Here I apply the term ‘symbiosis’ in the broad sense, following De Bary 1879 as “the living together of two differently named organisms“ independent of effects on the organisms involved (De Bary 1879), i.e. the biological interaction between the host and its symbionts may include mutualism, parasitism and commensalism. References Correa, C. C., and Ballard, J. W. O. (2016). Wolbachia associations with insects: winning or losing against a master manipulator. Frontiers in Ecology and Evolution, 3, 153. doi: https://doi.org/10.3389/fevo.2015.00153 De Bary, A. (1879). Die Erscheinung der Symbiose. Verlag von Karl J. Trubner, Strassburg. Engelstädter, J., and Hurst, G. D. (2009). The ecology and evolution of microbes that manipulate host reproduction. Annual Review of Ecology, Evolution, and Systematics, 40, 127-149. doi: https://doi.org/10.1146/annurev.ecolsys.110308.120206 Ezenwa, V. O., Gerardo, N. M., Inouye, D. W., Medina, M., and Xavier, J. B. (2012). Animal behavior and the microbiome. Science, 338(6104), 198-199. doi: https://doi.org/10.1126/science.1227412 Gurevich, Y., Lewin-Epstein, O., and Hadany, L. (2020). The evolution of paternal care: a role for microbes?. Philosophical Transactions of the Royal Society B, 375(1808), 20190599. doi: https://doi.org/10.1098/rstb.2019.0599 Hird, S. M. (2017). Evolutionary biology needs wild microbiomes. Frontiers in microbiology, 8, 725. doi: https://doi.org/10.3389/fmicb.2017.00725 Johnson, K. V. A., and Foster, K. R. (2018). Why does the microbiome affect behaviour?. Nature reviews microbiology, 16(10), 647-655. doi: https://doi.org/10.1038/s41579-018-0014-3 Kramer et al. (2017). When earwig mothers do not care to share: parent–offspring competition and the evolution of family life. Functional Ecology, 31(11), 2098-2107. doi: https://doi.org/10.1111/1365-2435.12915 Lewin-Epstein, O., Aharonov, R., and Hadany, L. (2017). Microbes can help explain the evolution of host altruism. Nature communications, 8(1), 1-7. doi: https://doi.org/10.1038/ncomms14040 Meunier, J., and Kölliker, M. (2012). Parental antagonism and parent–offspring co-adaptation interact to shape family life. Proceedings of the Royal Society B: Biological Sciences, 279(1744), 3981-3988. doi: https://doi.org/10.1098/rspb.2012.1416 Meunier, J., Wong, J. W., Gómez, Y., Kuttler, S., Röllin, L., Stucki, D., and Kölliker, M. (2012). One clutch or two clutches? Fitness correlates of coexisting alternative female life-histories in the European earwig. Evolutionary Ecology, 26(3), 669-682. doi: https://doi.org/10.1007/s10682-011-9510-x Nalepa, C. A. (2020). Origin of mutualism between termites and flagellated gut protists: transition from horizontal to vertical transmission. Frontiers in Ecology and Evolution, 8, 14. doi: https://doi.org/10.3389/fevo.2020.00014 Ratz, T., Kramer, J., Veuille, M., and Meunier, J. (2016). The population determines whether and how life-history traits vary between reproductive events in an insect with maternal care. Oecologia, 182(2), 443-452. doi: https://doi.org/10.1007/s00442-016-3685-3 Van Meyel, S., Devers, S., Dupont, S., Dedeine, F. and Meunier, J. (2021) Alteration of gut microbiota with a broad-spectrum antibiotic does not impair maternal care in the European earwig. bioRxiv, 2020.10.08.331363. ver. 5 peer-reviewed and recommended by PCI Evol Biol. https://doi.org/10.1101/2020.10.08.331363 | Alteration of gut microbiota with a broad-spectrum antibiotic does not impair maternal care in the European earwig | Sophie Van Meyel, Séverine Devers, Simon Dupont, Franck Dedeine and Joël Meunier | <p>The microbes residing within the gut of an animal host often increase their own fitness by modifying their host’s physiological, reproductive, and behavioural functions. Whereas recent studies suggest that they may also shape host sociality and... | Behavior & Social Evolution, Evolutionary Ecology, Experimental Evolution, Life History, Species interactions | Trine Bilde | 2020-10-09 14:07:47 | View | ||
10 Jul 2019
Population genomics supports clonal reproduction and multiple gains and losses of parasitic abilities in the most devastating nematode plant pestGeorgios D. Koutsovoulos, Eder Marques, Marie-Jeanne Arguel, Laurent Duret, Andressa C.Z. Machado, Regina M.D.G. Carneiro, Djampa K. Kozlowski, Marc Bailly-Bechet, Philippe Castagnone-Sereno, Erika V.S. Albuquerque, Etienne G.J. Danchin https://doi.org/10.1101/362129The scandalous pestRecommended by Nicolas Galtier based on reviews by 2 anonymous reviewersKoutsovoulos et al. [1] have generated and analysed the first population genomic dataset in root-knot nematode Meloidogyne incognita. Why is this interesting? For two major reasons. First, M. incognita has been documented to be apomictic, i.e., to lack any form of sex. This is a trait of major evolutionary importance, with implications on species adaptive potential. The study of genome evolution in asexuals is fascinating and has the potential to inform on the forces governing the evolution of sex and recombination. Even small amounts of sex, however, are sufficient to restore most of the population genetic properties of true sexuals [2]. Because rare events of sex can remain undetected in the field, to confirm asexuality in M. incognita using genomic data is an important step. The second reason why M. incognita is of interest is that this nematode is one of the most harmful pests currently living on earth. M. incognita feeds on the roots of many cultivated plants, including tomato, bean, and cotton, and has been of major agricultural importance for decades. A number of races were defined based on host specificity. These have played a key role in attempts to control the dynamic of M. incognita populations via crop rotations. Races and management strategies so far lack any genetic basis, hence the second major interest of this study. References [1] Koutsovoulos, G. D., Marques, E., Arguel, M. J., Duret, L., Machado, A. C. Z., Carneiro, R. M. D. G., Kozlowski, D. K., Bailly-Bechet, M., Castagnone-Sereno, P., Albuquerque, E. V., & Danchin, E. G. J. (2019). Population genomics supports clonal reproduction and multiple gains and losses of parasitic abilities in the most devastating nematode plant pest. bioRxiv, 362129, ver. 5, peer-reviewed and recommended by Peer Community in Evolutionary Biology. doi: 10.1101/362129 | Population genomics supports clonal reproduction and multiple gains and losses of parasitic abilities in the most devastating nematode plant pest | Georgios D. Koutsovoulos, Eder Marques, Marie-Jeanne Arguel, Laurent Duret, Andressa C.Z. Machado, Regina M.D.G. Carneiro, Djampa K. Kozlowski, Marc Bailly-Bechet, Philippe Castagnone-Sereno, Erika V.S. Albuquerque, Etienne G.J. Danchin | <p>The most devastating nematodes to worldwide agriculture are the root-knot nematodes with Meloidogyne incognita being the most widely distributed and damaging species. This parasitic and ecological success seem surprising given its supposed obli... | Adaptation, Bioinformatics & Computational Biology, Evolutionary Ecology, Genome Evolution, Genotype-Phenotype, Molecular Evolution, Phylogenetics / Phylogenomics, Population Genetics / Genomics, Reproduction and Sex | Nicolas Galtier | 2018-08-24 09:02:33 | View | ||
21 Feb 2023
Wolbachia genomics reveals a potential for a nutrition-based symbiosis in blood-sucking Triatomine bugsJonathan Filée, Kenny Agésilas-Lequeux, Laurie Lacquehay, Jean Michel Bérenger, Lise Dupont, Vagner Mendonça, João Aristeu da Rosa, Myriam Harry https://doi.org/10.1101/2022.09.06.506778Nutritional symbioses in triatomines: who is playing?Recommended by Natacha Kremer based on reviews by Alejandro Manzano Marín and Olivier DuronNearly 8 million people are suffering from Chagas disease in the Americas. The etiological agent, Trypanosoma cruzi, is mainly transmitted by triatomine bugs, also known as kissing or vampire bugs, which suck blood and transmit the parasite through their feces. Among these triatomine species, Rhodnius prolixus is considered the main vector, and many studies have focused on characterizing its biology, physiology, ecology and evolution. Interestingly, given that Rhodnius species feed almost exclusively on blood, their diet is unbalanced, and the insects can lack nutrients and vitamins that they cannot synthetize themself, such as B-vitamins. In all insects feeding exclusively on blood, symbioses with microbes producing B-vitamins (mainly biotin, riboflavin and folate) have been widely described (see review in Duron and Gottlieb 2020) and are critical for insect development and reproduction. These co-evolved relationships between blood feeders and nutritional symbionts could now be considered to develop new control methods, by targeting the ‘Achille’s heel’ of the symbiotic association (i.e., transfer of nutrient and / or control of nutritional symbiont density). But for this, it is necessary to better characterize the relationships between triatomines and their symbionts. R. prolixus is known to be associated with several symbionts. The extracellular gut symbiont Rhodococcus rhodnii, which reaches high bacterial densities and is almost fixed in R. prolixus populations, appears to be a nutritional symbiont under many blood sources. This symbiont can provide B-vitamins such as biotin (B7), niacin (B3), thiamin (B1), pyridoxin (B6) or riboflavin (B2) and can play an important role in the development and the reproduction of R. prolixus (Pachebat et al. (2013) and see review in Salcedo-Porras et al. (2020)). This symbiont is orally acquired through egg smearing, ensuring the fidelity of transmission of the symbiont from mother to offspring. However, as recently highlighted by Tobias et al. (2020) and Gilliland et al. (2022), other gut microbes could also participate to the provision of B-vitamins, and R. rhodnii could additionally provide metabolites (other than B-vitamins) increasing bug fitness. In the study from Filée et al., the authors focused on Wolbachia, an intracellular, maternally inherited bacterium, known to be a nutritional symbiont in other blood-sucking insects such as bedbugs (Nikoh et al. 2014), and its potential role in vitamin provision in triatomine bugs. After screening 17 different triatomine species from the 3 phylogenetic groups prolixus, pallescens and pictipes, they first show that Wolbachia symbionts are widely distributed in the different Rhodnius species. Contrary to R. rhodnii that were detected in all samples, Wolbachia prevalence was patchy and rarely fixed. The authors then sequenced, assembled, and compared 13 Wolbachia genomes from the infected Rhodnius species. They showed that all Wolbachia are phylogenetically positioned in the supergroup F that contains wCle (the Wolbachia from bedbugs). In addition, 8 Wolbachia strains (out of 12) encode a biotin operon under strong purifying selection, suggesting the preservation of the biological function and the metabolic potential of Wolbachia to supplement biotin in their Rhodnius host. From the study of insect genomes, the authors also evidenced several horizontal transfers of genes from Wolbachia to Rhodnius genomes, which suggests a complex evolutionary interplay between vampire bugs and their intracellular symbiont. This nice piece of work thus provides valuable information to the fields of multiple partners / nutritional symbioses and Wolbachia research. Dual symbioses described in insects feeding on unbalanced diets generally highlight a certain complementarity between symbionts that ensure the whole nutritional complementation. The study presented by Filée et al. leads rather to consider the impact of multiple symbionts with different lifestyles and transmission modes in the provision of a specific nutritional benefit (here, biotin). Because of the low prevalence of Wolbachia in certain species, a “ménage à trois” scenario would rather be replaced by an “open couple”, where the host relationship with new symbiotic partners (more or less stable at the evolutionary timescale) could provide benefits in certain ecological situations. The results also support the potential for Wolbachia to evolve rapidly along a continuum between parasitism and mutualism, by acquiring operons encoding critical pathways of vitamin biosynthesis. References Duron O. and Gottlieb Y. (2020) Convergence of Nutritional Symbioses in Obligate Blood Feeders. Trends in Parasitology 36(10):816-825. https://doi.org/10.1016/j.pt.2020.07.007 Filée J., Agésilas-Lequeux K., Lacquehay L., Bérenger J.-M., Dupont L., Mendonça V., Aristeu da Rosa J. and Harry M. (2023) Wolbachia genomics reveals a potential for a nutrition-based symbiosis in blood-sucking Triatomine bugs. bioRxiv, 2022.09.06.506778, ver. 3 peer-reviewed and recommended by Peer Community in Evolutionary Biology. https://doi.org/10.1101/2022.09.06.506778 Gilliland C.A. et al. (2022) Using axenic and gnotobiotic insects to examine the role of different microbes on the development and reproduction of the kissing bug Rhodnius prolixus (Hemiptera: Reduviidae). Molecular Ecology. https://doi.org/10.1111/mec.16800 Nikoh et al. (2014) Evolutionary origin of insect–Wolbachia nutritional mutualism. PNAS. 111(28):10257-10262. https://doi.org/10.1073/pnas.1409284111 Pachebat, J.A. et al. (2013). Draft genome sequence of Rhodococcus rhodnii strain LMG5362, a symbiont of Rhodnius prolixus (Hemiptera, Reduviidae, Triatominae), the principle vector of Trypanosoma cruzi. Genome Announc. 1(3):e00329-13. https://doi.org/10.1128/genomea.00329-13 Salcedo-Porras N., et al. (2020). The role of bacterial symbionts in Triatomines: an evolutionary perspective. Microorganisms. 8:1438. https://doi.org/10.3390%2Fmicroorganisms8091438 Tobias N.J., Eberhard F.E., Guarneri A.A. (2020) Enzymatic biosynthesis of B-complex vitamins is supplied by diverse microbiota in the Rhodnius prolixus anterior midgut following Trypanosoma cruzi infection. Computational and Structural Biotechnology Journal. 3395-3401. https://doi.org/10.1016/j.csbj.2020.10.031 | Wolbachia genomics reveals a potential for a nutrition-based symbiosis in blood-sucking Triatomine bugs | Jonathan Filée, Kenny Agésilas-Lequeux, Laurie Lacquehay, Jean Michel Bérenger, Lise Dupont, Vagner Mendonça, João Aristeu da Rosa, Myriam Harry | <p>The nutritional symbiosis promoted by bacteria is a key determinant for adaptation and evolution of many insect lineages. A complex form of nutritional mutualism that arose in blood-sucking insects critically depends on diverse bacterial symbio... | Genome Evolution, Phylogenetics / Phylogenomics, Species interactions | Natacha Kremer | Alejandro Manzano Marín | 2022-09-13 17:36:46 | View |
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